Developmental and comparative aspects of brine shrimp tubulin
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Genomic organization and developmental pattern of expression of the engrailed gene from the brine shrimp Artemia.
We report the isolation and characterization of an engrailed gene in the crustacean Artemia franciscana. The Artemia gene spans a genomic region of 15 kilobases and the coding sequence is interrupted by two introns. It appears to be the only gene of the engrailed family present in the Artemia genome. The predicted engrailed-like protein is 349 amino acids long and contains several domains inclu...
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Changes in the amounts of acid-soluble nucleotides during embryonic development of the encysted egg of the brine shrimp, Artemia salina, have been determined. Significant quantities of diguanosine tetraphosphate, the most abundant compound in the nucleotide pool, are apparently not utilized until hatching, at which time the amount falls precipitously. Diguanosine triphosphate, a homologue of th...
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N ucleic a c id m etabolism of th e b r in e shrim p, Artem ia s a l i n a , has been th e s u b je c t o f r e c e n t in v e s t ig a t io n a t th e Oak Ridge N a tio n a l L a b o ra to r ie s (U .S .A .) t o determ ine th e source o f n u c le ic a c id p u rin e s r e l a t i v e t o th e c e l l u l a r n u c le o tid e p oo ls d u rin g developm ent. As a seq u e l t o t h i s work, and...
متن کاملPurification and characterization of phenoloxidase from brine shrimp Artemia sinica.
Phenoloxidase from Artemia sinica (AsPO) was purified by Superdex 200 gel-filtration and Q Sepharose fast flow ion-exchange chromatography, and its properties were characterized biochemically and enzymatically by using L-dihydroxyphenylalanine (L-DOPA) as the specific substrate. Results showed that AsPO was isolated as a monomeric protein of 125.5 kDa in molecular mass. The optimal pH value and...
متن کاملEvaluation of the cytotoxic activity of crocin and safranal using potato disc and brine shrimp assays
Introduction: In this study, two constituents of saffron stigma (crocin and safranal) were tested for possible cytotoxic and antitumoral activity using brine shrimp and potato disc assays. Methods: In the brine shrimp assay, three wells containing 10 nauplii were tested for each concentration. After 24h, the number of the nauplii was counted. To investigate the antimicrobial activity of safr...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1984
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2190137